2021/07/15 by Helaina R. Stergas, Zoë Kalbag, Riley M. St. Clair +3
Biochemistry, Genetics and Molecular Biology · Neuroscience · #Microtubule and mitosis dynamics #Neurogenesis and neuroplasticity mechanisms #Retinal Development and Disorders
paper · doi:10.1002/dvdy.402
openalex publication_date 2021/07/15 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
BACKGROUND: The development of the central nervous system (CNS) requires critical cell signaling molecules to coordinate cell proliferation and migration in order to structure the adult tissue. Chicken tumor virus #10 Regulator of Kinase (CRK) and CRK-like (CRKL) are adaptor proteins with pre-metazoan ancestry and are known to be required for patterning laminated structures downstream of Reelin (RELN), such as the cerebral cortex, cerebellum, and hippocampus. CRK and CRKL also play crucial roles in a variety of other growth factor and extracellular matrix signaling cascades. The neuronal retina is another highly laminated structure within the CNS that is dependent on migration for proper development, but the cell signaling mechanisms behind neuronal positioning in the retina are only partly understood. RESULTS: We find that crk and crkl have largely overlapping expression within the developing zebrafish nervous system. We find that their disruption results in smaller eye size and loss of retinal lamination. CONCLUSIONS: Our data indicate that Crk adaptors are critical for proper development of the zebrafish neural retina in a crk/crkl dose-dependent manner.