2010/07/27 by A.L. Kamble, Ashwini Kamble, V.S. Meena +3
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · #Enzyme Catalysis and Immobilization #Enzyme Production and Characterization #Tannin, Tannase and Anticancer Activities
paper · doi:10.1111/j.1472-765x.2010.02909.x
openalex publication_date 2010/07/27 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
AIMS: To evaluate the effect of different physicochemical parameters such as agitation, aeration and pH on the growth and nitrile hydratase production by Rhodococcus erythropolis MTCC 1526 in a stirred tank reactor. METHODS AND RESULTS: Rhodococcus erythropolis MTCC 1526 was grown in 7-l reactor at different agitation, aeration and controlled pH. The optimum conditions for batch cultivation in the reactor were an agitation rate of 200 rev min(-1) , aeration 0.5 v/v/m at controlled pH 8. In this condition, the increase in nitrile hydratase activity was almost threefold compared to that in the shake flask. CONCLUSION: Agitation and aeration rate affected the dissolved-oxygen concentration in the reactor which in turn affected the growth and enzyme production. SIGNIFICANCE AND IMPACT OF THE STUDY: Cultivation of R. erythropolis MTCC 1526 in the reactor was found to have significant effect on the growth and nitrile hydratase production when compared to the shake flask.