1999/07/01 by Christophe Soumet, C. Soumet, Gwennola Ermel +8
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Listeria monocytogenes in Food Safety #Salmonella and Campylobacter epidemiology #Vibrio bacteria research studies
paper · doi:10.1046/j.1365-2672.1999.00559.x
openalex publication_date 1999/07/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/30
A multiplex-PCR-based assay (m-PCR) was developed for the detection of Salmonella and for the identification of the two serotypes Enteritidis and Typhimurium. Three sets of primers selected from different genomic sequences amplified a 429 bp fragment specific for the genus Salmonella within a randomly cloned sequence, a 559 bp target specific for Salmonella Typhimurium within the fliC gene and a 312 bp fragment specific for Salmonella Enteritidis within the sefA gene. The m-PCR-based assay was used for detecting Salmonella from 1078 environmental swabs of poultry houses. Prior to PCR, these swabs were pre-enriched in phosphate-buffered peptone water for 18-20 h and then sub-cultured on a Modified Semi-solid Rappaport Vassiliadis medium (MSRV) for 18-20 h. The m-PCR combined with MSRV had a better sensitivity (95%) than the bacteriological method (92.5%). The MSRV-m-PCR assay and the bacteriological method had an agreement rate of 95.6%.