2024/10/25 by Cécile Guinet, Marius Buronfosse, Philippe Tanguay +2 · 1 voice
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Environmental Science · #Bioenergy crop production and management #Forest Insect Ecology and Management #Yeasts and Rust Fungi Studies
paper · doi:10.1094/pdis-07-24-1477-sc
openalex publication_date 2024/10/25 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22
Melampsora medusae f. sp. tremuloidae is a quarantine organism for the European Union. In North America, this fungus causes rust disease on Populus tremuloides. In Europe, Populus tremula, an aspen closely related to P. tremuloides, is widespread and plays an important ecological role. Introduction of M. medusae f. sp. tremuloidae into Europe could be a major risk if this forma specialis could evolve and become virulent on P. tremula. To date, no PCR-based assay exists to specifically detect M. medusae f. sp. tremuloidae. In this study, a sensitive and specific real-time PCR assay has been developed based on the 28S rDNA. The assay proved to be reliable using many real-time PCR kits and platforms. It can be used to monitor the introduction and the spread of M. medusae f. sp. tremuloidae in the context of phytosanitary regulations.