2026/04/17 by Minli Ruan, Sean M. Engels, Matthew R. Burroughs +13 · 1 voice · 1 citation
Biochemistry, Genetics and Molecular Biology · #Biochemical and Molecular Research #Cancer, Hypoxia, and Metabolism #RNA modifications and cancer
paper · doi:10.1038/s41467-026-71654-y
openalex publication_date 2026/04/17 · openalex created_date 2026/04/18 · openalex updated_date 2026/08/01
Pseudouridine (Ψ) is an abundant post-transcriptional modification found across all classes of RNA. It is widely speculated that Ψ inclusion in messenger RNAs (mRNAs) might provide an avenue for cells to control gene expression post-transcriptionally. Here we demonstrate that one of the principal mRNA pseudouridylating enzymes, pseudouridine synthase 7 (PUS7), exhibits a stress-induced accumulation in the cytoplasm of yeast and human epithelial lung cells. Stress-induced and cytoplasmic localization of PUS7 promotes Ψ-incorporation into hundreds of mRNA targets. In contrast, the modification status of tRNA sites targeted by PUS7 (Ψ13 and Ψ35) is unperturbed. Furthermore, engineered PUS7 cytoplasmic localization increases cellular fitness under reactive oxygen species (ROS) and divalent metal ion stress. Quantitative proteomics reveal a reshaping of the proteome upon PUS7 relocalization under stress. Collectively, our data demonstrate that PUS7 localization alters mRNA pseudouridylation patterns, reshapes the proteome, and influences cellular fitness. Pseudouridine is a widespread RNA modification implicated in the regulation of gene expression. Here, authors show that PUS7 relocalization changes mRNA pseudouridylation, reshapes the proteome, and enhances cell fitness.