2020/07/22 by Maxime JY Zimmermann, André Maia Chagas, Philipp Bartel +3 · 1 voice
Biochemistry, Genetics and Molecular Biology · Neuroscience · #Advanced Fluorescence Microscopy Techniques #Photoreceptor and optogenetics research #Retinal Development and Disorders
paper · doi:10.1016/j.ohx.2020.e00127
openalex publication_date 2020/07/22 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
Two-photon (2P) microscopy is a cornerstone technique in neuroscience research. However, combining 2P imaging with spectrally arbitrary light stimulation can be challenging due to crosstalk between stimulation light and fluorescence detection. To overcome this limitation, we present a simple and low-cost electronic solution based on an ESP32 microcontroller and a TLC5947 LED driver to rapidly time-interleave stimulation and detection epochs during scans. Implemented for less than 100, our design can independently drive up to 24 arbitrary spectrum LEDs to meet user requirements. We demonstrate the utility of our stimulator for colour vision experiments on the in vivo tetrachromatic zebrafish retina and for optogenetic circuit mapping in Drosophila .