2017/07/12 by Jeanette E.L. Carlsson, Jeanette E. L. Carlsson, Damian Egan +4 · 1 citation
Environmental Science · #Aquatic Invertebrate Ecology and Behavior #Environmental DNA in Biodiversity Studies #Fish Ecology and Management Studies
paper · doi:10.1002/aqc.2788
openalex publication_date 2017/07/12 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/30
Abstract 1. Environmental (e)DNA assays are becoming increasingly used to detect rare or invasive aquatic species. 2. The Critically Endangered freshwater pearl mussel Margaritifera margaritifera is undergoing range‐wide reduction in population numbers and distribution. 3. An eDNA assay to detect the presence of M. margaritifera was developed, based on the mitochondrial cytochrome oxidase I gene, utilizing species‐specific primers, a minor groove binding (MGB) probe and quantitative (q)PCR approaches. 4. The results from this pilot study demonstrated high sensitivity both in laboratory and field trials, and provide a valuable non‐invasive tool for detecting M. margaritifera .