2022/11/04 by S. Khavnekar, Sagar Khavnekar, William Wan +9
Biochemistry, Genetics and Molecular Biology · Materials Science · Physics and Astronomy · #Advanced Electron Microscopy Techniques and Applications #Advanced X-ray Imaging Techniques #Electron and X-Ray Spectroscopy Techniques
paper · doi:10.1016/j.jsb.2022.107911
openalex publication_date 2022/11/04 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/30
Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) can resolve protein complexes at near atomic resolution, and when combined with focused ion beam (FIB) milling, macromolecules can be observed within their native context. Unlike single particle acquisition (SPA), cryo-ET can be slow, which may reduce overall project throughput. We here propose a fast, multi-position tomographic acquisition scheme based on beam-tilt corrected beam-shift imaging along the tilt axis, which yields sub-nanometer in situ STA averages.