PMN recruitment in inflammatory lung injury models follows classical transendothelial migration paradigms requiring PECAM-1 and CD99

2025/05/30 by Maureen E. Haynes, Erika Arias, David P. Sullivan +2
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · #Extracellular vesicles in disease #Immune Response and Inflammation #Immune cells in cancer

paper · doi:10.1152/ajplung.00069.2025

openalex publication_date 2025/05/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/24

Abstract

Previous studies have shown that neutrophil extravasation in the lung was selectin-independent and the requirement for leukocyte integrins was stimulus-dependent. This study demonstrates that PECAM-1 and CD99 are required for PMN transmigration during chemical, bacterial, and ischemia/reperfusion lung inflammation. We show directly in real time, using intravital microscopy, that neutrophils extravasate from alveolar capillaries. Blocking antibodies against PECAM-1 or CD99 prevented transmigration into the lung airspace, just as they prevent transmigration in the systemic circulation.

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