2018/10/04 by Andrea Porzionato, Patrizia Zaramella, Arben Dedja +9
Biochemistry, Genetics and Molecular Biology · Medicine · #Congenital Diaphragmatic Hernia Studies #Extracellular vesicles in disease #Neonatal Respiratory Health Research
paper · doi:10.1152/ajplung.00109.2018
openalex publication_date 2018/10/04 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Mesenchymal stem cells (MSCs) prevent the onset of bronchopulmonary dysplasia (BPD) in animal models, an effect that seems to be mediated by their secreted extracellular vesicles (EVs). The aim of this study was to compare the protective effects of intratracheally (IT) administered MSCs versus MSC-EVs in a hyperoxia-induced rat model of BPD. At birth, rats were distributed as follows: animals raised in ambient air for 2 wk ( n = 10), and animals exposed to 60% oxygen for 2 wk and treated with IT-administered physiological solution ( n = 10), MSCs ( n = 10), or MSC-EVs ( n = 10) on postnatal days 3, 7, and 10. The sham-treated hyperoxia-exposed animals showed reductions in total surface area of alveolar air spaces, and total number of alveoli ( N alv ), and an increased mean alveolar volume (V alv ). EVs prompted a significant increase in N alv ( P < 0.01) and a significant decrease in V alv ( P < 0.05) compared with sham-treated animals, whereas MSCs only significantly improved N alv ( P < 0.05). Small pulmonary vessels of the sham-treated hyperoxia-exposed rats also showed an increase in medial thickness, which only EVs succeeded in preventing significantly ( P < 0.05). In conclusion, both EVs and MSCs reduce hyperoxia-induced damage, with EVs obtaining better results in terms of alveolarization and lung vascularization parameters. This suggests that IT-administered EVs could be an effective approach to BPD treatment.