2026/06/01 by Anandi Bierman, Minette Karsten, Annelie Smit +3
paper · doi:10.17159/2254-8854/2026/a25994
The rapid and accurate identification of invasive pest insects at quarantine stations or ports of entry is crucial for preventing their establishment and spread in new territories. Here, we present an effective and rapid genetic identification technique utilising loop-mediated isothermal amplification (LAMP) to detect the presence of the peach fruit fly, Bactrocera zonata. The LAMP assay was designed to target the cytochrome oxidase subunit I (COI) gene region of B. zonata, ensuring high specificity and sensitivity, and to discriminate it from a range of other economically important Bactrocera species. By utilising isothermal conditions, LAMP eliminates the need for lengthy thermal cycling and enables rapid amplification of target DNA within a short period. Additionally, a rapid DNA extraction method combined with the use of an intercalating UV fluorescent dye allows for fast and reliable interpretation of results without the need for specialised equipment. Validation studies were conducted using known samples of B. zonata, as well as other Bactrocera species commonly encountered in quarantine settings including B. dorsalis, B. correcta, B. tryoni, B. oleae, B. cucurbitae and B. tau. The power of this LAMP assay lies in its specificity to accurately distinguish B. zonata from other closely related Bactrocera species. Moreover, the assay exhibits a remarkably short turnaround time, with results obtained in under an hour, facilitating timely decision-making at quarantine stations.