2004/02/01 by Z. Ma, Zhonghua Ma, Themis J. Michailides +1 · 1 citation
Agricultural and Biological Sciences · Chemistry · #Fungal Plant Pathogen Control #Garlic and Onion Studies #Synthesis and Biological Evaluation
paper · doi:10.1046/j.1439-0434.2003.00806.x
openalex publication_date 2004/02/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
Abstract Alternaria late blight caused by Alternaria spp. in the alternata, tenuissima and arborescens species‐groups is one of the most common fungal diseases of pistachio in California. A single point mutation resulting in the replacement of a glycine by an alanine at codon 143 (G143A) in the mitochondrial cytochrome b (cyt b ) has been found in all azoxystrobin‐resistant isolates of these three species from California pistachio. In this study, a pair of allele‐specific polymerase chain reaction (PCR) primers was developed to detect this point mutation. The allele‐specific PCR assay coupled with a rapid DNA extraction method could detect azoxystrobin‐resistant Alternaria isolates in a few hours. The allele‐specific PCR method was also reliable for detecting azoxystrobin‐resistant Alternaria directly in both laboratory‐inoculated and naturally infected pistachio leaves.