2004/12/01 by C. Gomez‐Machorro, K. E. Bennett, M. Del Lourdes Munoz +1
paper · doi:10.1111/j.0962-1075.2004.00522.x
Abstract Quantitative trait loci (QTL) affecting the ability of the Aedes aegypti midgut to become infected with Dengue 2 virus (DEN2) were mapped in the F 5 generation of an advanced intercross line (AIL). A strain of Ae. aegypti previously selected for DEN2 susceptibility was crossed to a new strain selected for refractoriness to midgut infection. In P 1 and F 1 parents and 147 F 5 offspring, genotypes at forty‐four cDNA loci were analysed. A new sex linked QTL and a second QTL on chromosome II with genotypes subject to balancing selection were detected that condition midgut susceptibility. Alleles at these QTL contributed additively in determining susceptibility and accounted for ∼24% of the phenotypic variance. Markers associated with a midgut escape barrier were inconsistently supported.