2011/10/01 by Duck-Jin Kim, Il Yung Sohn, Jin‐Heak Jung +8
Biochemistry, Genetics and Molecular Biology · Economics, Econometrics and Finance · Engineering · Materials Science · Social Sciences · #Advanced biosensing and bioanalysis techniques #Firm Innovation and Growth #Graphene and Nanomaterials Applications #Graphene research and applications #Polish socio-economic development #Regional Development and Policy
paper · doi:10.1016/j.bios.2012.09.040
openalex publication_date 2011/10/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/02
We report reduced graphene oxide field effect transistor (R-GO FET) biosensor for label-free ultrasensitive detection of a prostate cancer biomarker, prostate specific antigen/α1-antichymotrypsin (PSA-ACT) complex. The R-GO channel in the device was formed by reduction of graphene oxide nanosheets networked by a self-assembly process. Immunoreaction of PSA-ACT complexes with PSA monoclonal antibodies on the R-GO channel surface caused a linear response in the shift of the gate voltage, V(g,min), where the minimum conductivity occurs. The R-GO FET can detect protein-protein interactions down to femtomolar level with a dynamic range over 6-orders of magnitude in the V(g,min) shift as a sensitivity parameter. High association constants of 3.2 nM(-1) and 4.2 nM(-1) were obtained for the pH 6.2 and pH 7.4 analyte solutions, respectively. The R-GO FET biosensor showed a high specificity to other cancer biomarker in the phosphate buffered saline solutions as well as in the human serum.