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Antimicrobial Resistance, Virulence and Resistance Genes in Aeromonas From Diseased Fish in China's Major Freshwater Aquaculture Regions

2026/02/11 by Linying Liu, Peipei Yi, Yuhan Chen +6
Biochemistry, Genetics and Molecular Biology · Environmental Science · Immunology and Microbiology · #Antibiotic Resistance in Bacteria #Aquaculture disease management and microbiota #Pharmaceutical and Antibiotic Environmental Impacts

paper · pdf · doi:10.1111/jfd.70145

openalex publication_date 2026/02/11 · openalex created_date 2026/02/13 · openalex updated_date 2026/06/21

Abstract

ABSTRACT This study aimed to characterise Aeromonas isolates from diseased freshwater fish in Jiangxi Province, with a focus on their species distribution, genetic diversity, virulence and resistance gene profiles and antibiotic susceptibility. A total of 105 Aeromonas strains were isolated and identified, with Aeromonas veronii being the dominant species (68.6%). ERIC‐PCR divided the isolates into 76 molecular types. Most isolates carried multiple virulence genes. These isolates exhibited high resistance to ampicillin (98.1%), erythromycin (84.7%) and rifampicin (54.3%), but were susceptible to chloramphenicol, florfenicol and ceftriaxone. The overall multidrug‐resistance (MDR) rate was 64.8%. Aeromonas hydrophila showed a significantly higher MDR rate (95%) than both A. veronii and Aeromonas jandaei (each 58.3%). Resistance genes such as bla TEM , qnrA and qnrS were prevalent, while others, including tetA and sul1, were less common. Two class 1 integron gene cassettes were identified: ‘ drfB4‐catB3 ’ ( n = 16) and ‘ drfA17‐aadA5 ’ ( n = 1). Aeromonas isolates in Jiangxi exhibit high genetic diversity, significant virulence potential, andconcerning antibiotic resistance profiles. Of particular concern is A. hydrophila , which poses a high multidrug‐resistance risk.

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