2019/05/07 by Tijana Vasiljevic, Tijana Vasiljević, Germán Augusto Gómez‐Ríos +4
Chemistry · Pharmacology, Toxicology and Pharmaceutics · #Analytical chemistry methods development #Forensic Toxicology and Drug Analysis #Mass Spectrometry Techniques and Applications
paper · doi:10.1002/rcm.8477
openalex publication_date 2019/05/07 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
Rationale The workload of clinical laboratories has been steadily increasing over the last few years. High‐throughput (HT) sample processing allows scientists to spend more time undertaking matters of critical thinking rather than laborious sample processing. Herein we introduce a HT 96‐solid‐phase microextraction (SPME) transmission mode (TM) system coupled to direct analysis in real time (DART) mass spectrometry (MS). Methods Model compounds (opioids) were extracted from urine and plasma samples using a 96‐SPME‐TM device. A standard voltage and pressure (SVP) DART source was used for all experiments. Examination of SPME‐TM performance was done using high‐resolution mass spectrometry (HRMS) in full scan mode (100–500 m/z ), whereas quantitation of opioids was performed using triple quadrupole MS in multiple reaction monitoring mode and by using a matrix‐matched internal standard correction method. Results Thirteen points (0.5 to 200 ng mL −1 ) were used to establish a calibration curve. Low limits of quantitation (LOQ) were obtained (0.5 to 25 ng mL −1 ) for matrices used. Acceptable accuracy (71.4–129.4%) and repeatability (1.1–24%) were obtained for validation levels tested (0.5, 30 and 90 ng mL −1 ). In less than 1.5 hours, 96 samples were extracted, desorbed and processed using the 96‐SPME‐TM system coupled to DART‐MS. Conclusions A rapid HT method for detection of opioids in urine and plasma samples was developed. This study demonstrated that ambient ionization mass spectrometry coupled to robust sample preparation methods such as SPME‐TM can rapidly and efficiently screen/quantify target analytes in a HT context.