2015/02/10 by Xiankun Zeng, Steven X. Hou · 3 citations
Agricultural and Biological Sciences · Immunology and Microbiology · Neuroscience · #Insect Utilization and Effects #Invertebrate Immune Response Mechanisms #Neurobiology and Insect Physiology Research
paper · doi:10.1242/dev.113357
openalex publication_date 2015/02/10 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/31
Functional mature cells are continually replenished by stem cells to maintain tissue homoeostasis. In the adult Drosophila posterior midgut, both terminally differentiated enterocyte (EC) and enteroendocrine (EE) cells are generated from an intestinal stem cell (ISC). However, it is not clear how the two differentiated cells are generated from the ISC. In this study, we found that only ECs are generated through the Su(H)GBE(+) immature progenitor enteroblasts (EBs), whereas EEs are generated from ISCs through a distinct progenitor pre-EE by a novel lineage-tracing system. EEs can be generated from ISCs in three ways: an ISC becoming an EE, an ISC becoming a new ISC and an EE through asymmetric division, or an ISC becoming two EEs through symmetric division. We further identified that the transcriptional factor Prospero (Pros) regulates ISC commitment to EEs. Our data provide direct evidence that different differentiated cells are generated by different modes of stem cell lineage specification within the same tissues.