2022/07/19 by Philip Prins, Timothy D. Noakes, Timothy Noakes +14
Biochemistry, Genetics and Molecular Biology · Medicine · #Diet and metabolism studies #Muscle metabolism and nutrition #Sports Performance and Training
paper · pdf · doi:10.5114/biolsport.2023.116452
openalex publication_date 2022/07/19 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Recently we reported similar performances in both progressive tests to exhaustion (VO 2 max) and 5km running time trials (5KTT) after consuming low-carbohydrate, high-fat (LCHF) or high-carbohydrate, low-fat (HCLF) diets. Accordingly, we tested the null hypothesis that the metabolic responses during both tests would be similar across diets. In a randomized, counterbalanced, cross-over design, seven male athletes (VO 2 max: 61.9 6.1 mL/kg/min; age: 35.6 8.4 years; height: 178.7 4.1 cm; mass: 68.6 1.6 kg; body fat: 5.0 1.3%) completed six weeks of LCHF (6/69/25% energy carbohydrate/fat/protein) and HCLF (57/28/15% energy carbohydrate/fat/protein) diets, separated by a two-week washout. Substrate utilization and energy expenditure were measured during VO 2 max tests and 5KTTs. The LCHF diet markedly increased fat oxidation and reduced carbohydrate oxidation, with no associated impairment in either the VO 2 max tests or the 5KTTs. Following the LCHF diet, athletes generated 50% or more of their energy requirements from fat at exercise intensities up to 90% VO 2 max and reached the crossover point for substrate utilization at ~85% VO 2 max. In contrast, following the HCLF diet, carbohydrate provided more than 50% of the total energy consumption at all exercise intensities. During the 5KTT, ~56% of energy was derived from fat following the LCHF diet whereas more than 93% of the energy came from carbohydrate following the HCLF diet. This study provides evidence of greater metabolic flexibility following LCHF eating and challenges the popular doctrines of "carbohydrate dependence" for high intensity exercise and the role dietary macronutrients play in human performance.