2016/02/29 by Hiroshi Higuchi, Toru Shoji, Shinji Iijima +2
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · Medicine · #Chemokine receptors and signaling #Glycosylation and Glycoproteins Research #Immune Response and Inflammation
paper · pdf · doi:10.1080/09168451.2016.1146070
openalex publication_date 2016/02/29 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Siglecs recognize the sialic acid moiety and regulate various immune responses. In the present study, we compared the expression levels of Siglecs in human monocytes and macrophages using a quantitative real-time reverse transcription-polymerase chain reaction analysis. The differentiation of monocytes into macrophages by macrophage colony-stimulating factor or granulocyte macrophage colony-stimulating factor enhanced the expression of Siglec-7 and Siglec-9. The differentiated macrophages were stimulated by lipopolysaccharide (LPS) plus interferon (IFN)-γ or interleukin (IL)-4. The expression of Siglec-10 was enhanced by IL-4, whereas that of Siglec-7 was reduced by LPS plus IFN-γ. The expression of Siglec-9 was not affected by these stimuli. The knockdown of Siglec-9 enhanced the expression of CCR7 induced by the LPS or the LPS plus IFN-γ stimulation, and decreased the IL-4-induced expression of CD200R. These results suggest that Siglec-9 is one of the main Siglecs in human blood monocytes/macrophages and modulates innate immunity.