2012/05/23 by Takashi FUJIKI, Takashi Fujiki, Yoshitaka HIROSE +5 · 2 citations
Agricultural and Biological Sciences · Medicine · Nursing · #Infant Nutrition and Health #Probiotics and Fermented Foods #Viral gastroenteritis research and epidemiology
paper · pdf · doi:10.1271/bbb.110919
openalex publication_date 2012/05/23 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/30
This study reports the effect of heat treating Lactobacillus plantarum L-137 on its in vitro cytokine-inducing activity, on the stability of this activity in simulated digestive juices, and on its in vivo immunomodulatory properties. L-137 cells were harvested at the stationary phase with or without the subsequent heat treatment and then lyophilized. Heat-killed L-137 cells stimulated mouse spleen cells to produce more interleukin-12p40 than unheated L-137. The interleukin-12p40-inducing activity of unheated L-137 was significantly lower when incubated with simulated intestinal juice, but the activity of heat-killed L-137 cells was maintained. Furthermore, heat-killed L-137 was more protective than unheated L-137 in a mouse model of dextran sulfate sodium-induced colitis. A heat treatment may therefore be effective for enhancing the immunomodulatory activity of L-137 cells.