Chiral separation of underivatized amino acids in supercritical fluid chromatography with chiral crown ether derived column

2020/03/05 by Larry Miller, Lei Yue · 1 citation
Biochemistry, Genetics and Molecular Biology · Chemistry · #Analytical Chemistry and Chromatography #Mass Spectrometry Techniques and Applications #Metabolomics and Mass Spectrometry Studies

paper · doi:10.1002/chir.23204

openalex publication_date 2020/03/05 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

The supercritical fluid chromatographic separation of underivatized amino acids was explored using immobilized chiral crown ether column CROWNPAK CR-I (+) and mass spectrometric detection. The type of modifier, acidic additives, and the role of water were investigated. Enantioseparation was achieved for all 18 amino acids investigated with short retention times (less than 3 minutes) and average resolution of greater than 5.0. Analysis of enantiomerically pure standards demonstrated the D enantiomer eluted first for all amino acids using a CROWNPAK CR-I (+) column.

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• Method for the simultaneous enantioselective analysis of 21 natural amino acids and its application to analytics of fluorenylmethoxycarbonyl-derivatives under liquid chromatography conditions

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