A myocardin-adjacent lncRNA balances SRF-dependent gene transcription in the heart

2021/05/13 by Douglas M. Anderson, Kelly M. Anderson, Kelly Anderson +8
Biochemistry, Genetics and Molecular Biology · #Cancer-related molecular mechanisms research #RNA Research and Splicing #RNA modifications and cancer

paper · pdf · doi:10.1101/gad.348304.121

openalex publication_date 2021/05/13 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31

Abstract

Myocardin, a potent coactivator of serum response factor (SRF), competes with ternary complex factor (TCF) proteins for SRF binding to balance opposing mitogenic and myogenic gene programs in cardiac and smooth muscle. Here we identify a cardiac lncRNA transcribed adjacent to myocardin , named CARDINAL, which antagonizes SRF-dependent mitogenic gene transcription in the heart. CARDINAL -deficient mice show ectopic TCF/SRF-dependent mitogenic gene expression and decreased cardiac contractility in response to age and ischemic stress. CARDINAL forms a nuclear complex with SRF and inhibits TCF-mediated transactivation of the promitogenic gene c-fos, suggesting CARDINAL functions as an RNA cofactor for SRF in the heart.

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