Perspective: machines for RNAi

2005/03/01 by Yukihide Tomari, Phillip D. Zamore · 2 citations
Biochemistry, Genetics and Molecular Biology · #MicroRNA in disease regulation #RNA Interference and Gene Delivery #RNA Research and Splicing

paper · pdf · doi:10.1101/gad.1284105

openalex publication_date 2005/03/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01

Abstract

RNA silencing pathways convert the sequence information in long RNA, typically double-stranded RNA, into approximately 21-nt RNA signaling molecules such as small interfering RNAs (siRNAs) and microRNAs (miRNAs). siRNAs and miRNAs provide specificity to protein effector complexes that repress mRNA transcription or translation, or catalyze mRNA destruction. Here, we review our current understanding of how small RNAs are produced, how they are loaded into protein complexes, and how they repress gene expression.

Citations

• An Extensive Class of Small RNAs in Caenorhabditis elegans
• Regulation of Heterochromatic Silencing and Histone H3 Lysine-9 Methylation by RNAi
• Micro RNAs are complementary to 3′ UTR sequence motifs that mediate negative post-transcriptional regulation
• RITS acts in cis to promote RNA interference–mediated transcriptional and post-transcriptional silencing
• Establishment and Maintenance of a Heterochromatin Domain
• Identification of Novel Genes Coding for Small Expressed RNAs
• An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegans

Cited by

• High-Throughput Sequencing of Arabidopsis microRNAs: Evidence for Frequent Birth and Death of MIRNA Genes
• Pol II–Expressed shRNA Knocks Down Sod2 Gene Expression and Causes Phenotypes of the Gene Knockout in Mice

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• MicroRNA Systems Biology
• Small RNA driven feed-forward loop: Fine-tuning of protein synthesis through sRNA mediated cross-talk
• Kinetic modelling of competition and depletion of shared miRNAs by competing endogenous RNAs
• Posttranscriptional Upregulation by MicroRNAs