1997/10/01 by Evans S. Lagudah, Evans Lagudah, Odile Moullet +2 · 2 citations
Agricultural and Biological Sciences · #Entomopathogenic Microorganisms in Pest Control #Legume Nitrogen Fixing Symbiosis #Nematode management and characterization studies
paper · doi:10.1139/g97-087
openalex publication_date 1997/10/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/06/11
The Cre3 gene confers a high level of resistance to the root endoparasitic nematode Heterodera avenae in wheat. A DNA marker cosegregating with H. avenae resistance was used as an entry point for map-based cloning of a disease resistance gene family at the Cre3 locus. Two related gene sequences have been analysed at the Cre3 locus. One, identified as a cDNA clone, encodes a polypeptide with a nucleotide binding site (NBS) and a leucine-rich region; this member of the disease resistance gene family is expressed in roots. A second Cre3 gene sequence, cloned as genomic DNA, appears to be a pseudogene, with a frame shift caused by a deletion event. These two genes, related to members of the cytoplasmic NBS-leucine rich repeat class of plant disease resistance genes were physically mapped to the distal 0.06 fragment of the long arm of wheat chromosome 2D and cosegregated with nematode resistance.