1977/07/01 by Frank L. Graham, W. C. Russell, James R. Smiley +1 · 1 voice · 4 citations
Biochemistry, Genetics and Molecular Biology · #Virus-based gene therapy research #RNA Interference and Gene Delivery #Animal Genetics and Reproduction
paper · doi:10.1099/0022-1317-36-1-59
Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides synthesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in otheruman cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 250 K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.