2024/11/25 by Photini F. Rice, Photini Rice, Makenna Aitken +10
Biochemistry, Genetics and Molecular Biology · Engineering · #Advanced Fluorescence Microscopy Techniques #Artificial intelligence #Computer science #Modality (human–computer interaction) #Optical Coherence Tomography Applications #Optics #Photoacoustic and Ultrasonic Imaging #Physics #Window (computing)
paper · pdf · doi:10.1080/07366205.2024.2445452
openalex publication_date 2024/11/25 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/05
Current dorsal skin flap window chambers with flat glass windows are compatible with optical coherence tomography (OCT) and multiphoton microscopy (MPM) imaging. However, light sheet fluorescence microscopy (LSFM) performs best with a cylindrical or spherical sample located between its two 90° objectives and when all sample materials have the same index of refraction (n). A modified window chamber with a domed viewing window made from fluorinated ethylene propylene (FEP), with n similar to water and tissue, was designed. In vitro imaging of collagen gels and microsphere phantoms with and without the dome showed small decreases in signal strength and image resolution due to the dome. Using a custom mouse platform for stabilization and anesthesia support, in vivo multimodality imaging with OCT, MPM, and LSFI was demonstrated.