2011/02/01 by Seth W. Perry, John P. Norman, Justin Barbieri +2 · 2 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Engineering · #Biochemical engineering #Biochemistry #Biology #Biophysics #Computational biology #Computer science #Context (archaeology) #Engineering #Inner mitochondrial membrane #Membrane potential #Metabolism and Genetic Disorders #Mitochondrial Function and Pathology #Mitochondrion #Molecular Sensors and Ion Detection
paper · doi:10.2144/000113610
openalex publication_date 2011/02/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/04
Fluorescent probes for monitoring mitochondrial membrane potential are frequently used for assessing mitochondrial function, particularly in the context of cell fate determination in biological and biomedical research. However, valid interpretation of results obtained with such probes requires careful consideration of numerous controls, as well as possible effects of non-protonic charges on dye behavior. In this context, we provide an overview of some of the important technical considerations, controls, and parallel complementary assays that can be employed to help ensure appropriate interpretation of results, thus providing a practical usage guide for monitoring mitochondrial membrane potentials with cationic probes. In total, this review will help illustrate both the strengths and potential pitfalls of common mitochondrial membrane potential dyes, and highlight best-usage approaches for their efficacious application in life sciences research.