Method for Isolation of PCR-Ready Genomic DNA from Zebrafish Tissues

2007/11/01 by Nathan D. Meeker, Sarah A. Hutchinson, Linh Ho +1 · 1 citation
Biochemistry, Genetics and Molecular Biology · #Animal Genetics and Reproduction #Bioinformatics #Biology #CRISPR and Genetic Engineering #Computational biology #DNA #DNA extraction #Gene #Genetics #Isolation (microbiology) #Molecular biology #Polymerase chain reaction #Transgene #Zebrafish #Zebrafish Biomedical Research Applications #genomic DNA

paper · doi:10.2144/000112619

openalex publication_date 2007/11/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/05

Abstract

Here we describe a method for the isolation of PCR-ready genomic DNA from various zebrafish tissues that is based on a previously published murine protocol. The DNA solutions are of sufficient quality to allow PCR detection of transgenes from all commonly used zebrafish tissues. In sperm, transgene amplification was successful even when diluted 1000-fold, allowing easy identification of transgenic founders. Given its speed and low cost, we anticipate that the adoption of this method will streamline DNA isolation for zebrafish research.

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