2018/12/05 by Zhenguo Zeng, Dan Li, Fen Liu +7
paper · doi:10.1002/jcb.28142
Abstract The effects and mechanisms of mitochondrial DNA (mtDNA) in the development of sepsis‐induced lung injury is not well understood. In our present study, we studied the mtDNA effects in sepsis‐induced lung injury model, in vitro and in vivo. Compared with the Normal group, the lung histopathological score, the number of positive apoptosis cell, wet/dry ( W / D) ratio and TNF‐α, IL‐1β, and IL‐6 concentrations of lipopolysaccharides (LPSs) and mtDNA groups were significantly increased ( P W / D ratio, number of apoptosis cell and tumor necrosis factor‐α (TNF‐α), interleukin (IL)‐1β, and IL‐6 concentrations of LPS + mtDNA and small interfering RNA (siRNA)‐NC + LPS + mtDNA groups were significantly upregulated compared with those of LPS group ( P W / D ratio and TNF‐α, IL‐1β, and IL‐6 concentrations were significantly improved within the toll‐like receptor (TLR9)siRNA + LPS + mtDNA group compared with the LPS group ( P P P P