1993/03/01 by Zulkarnain Edward, Tsutomu Takegami
Immunology and Microbiology · Medicine · #HIV Research and Treatment #Mosquito-borne diseases and control #Virology and Viral Diseases
paper · pdf · doi:10.1111/j.1348-0421.1993.tb03206.x
openalex publication_date 1993/03/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Recently it has been reported that Japanese encephalitis virus (JEV)-specific RNAs can be synthesized in vitro in the subcellular fraction including outer-nuclear membrane (Takegami and Hotta, 1989). The results of Western blot analysis and indirect immunofluorescence test using two kinds of monospecific antisera against JEV nonstructural proteins NS3 and NS5 showed that NS3 and NS5 were membrane-associated proteins and formed the complex at the perinuclear site in the infected cells. Both antisera against NS3 and NS5 inhibited in vitro RNA synthesis. These results suggest that NS5 and NS3 play important role(s) in flavivirus RNA replication.