1993/06/01 by Yoichiro Kusunoki, Hua Huang, Yasuko Fukuda +4
Medicine · #Cytomegalovirus and herpesvirus research #Parvovirus B19 Infection Studies #Viral-associated cancers and disorders
paper · doi:10.1111/j.1348-0421.1993.tb03237.x
openalex publication_date 1993/06/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
A limiting dilution analysis was established to determine the precursor frequency (PF) of cytotoxic lymphocytes against autologous B cells transformed with the Epstein-Barr virus (EBV). This method was found to detect mainly self-restricted T-cell activity and little non-self-restricted cytotoxicity. The mean PF in 21 healthy EBV-seropositive persons was 1.4 x 10(-3) (range: 0.03 x 10(-3) to 8.7 x 10(-3)) for peripheral blood mononuclear cells, whereas 4 samples of mononuclear cells obtained from umbilical cord blood had PFs below 0.007 x 10(-3). A positive correlation was observed between the PF and serum antibody titers against EBV-associated nuclear antigen among the seropositive persons.