A stableEscherichia coli-mycobacteria shuttle vector âpSO246â inMycobacterium bovisBCG

1996/01/01 by Sohkichi Matsumoto, Mikio Tamaki, Hideharu Yukitake +4
Biochemistry, Genetics and Molecular Biology · Medicine · #Antibiotic Resistance in Bacteria #Mycobacterium research and diagnosis #Tuberculosis Research and Epidemiology

paper · pdf · doi:10.1111/j.1574-6968.1996.tb07995.x

openalex publication_date 1996/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29

Abstract

The most widely used plasmid vector system in mycobacteria is based on pAL5000 from Mycobacterium fortuitum. The derivatives of the pAL5000-based shuttle vectors between Escherichia coli and mycobacteria, which we have utilized to secrete recombinant antigens, were generated. The stability of the vectors was assessed in Mycobacterium bovis BCG (BCG). The plasmid vector pSO246 was stable in BCG for at least 50 generations.

Related

• Small, Stable Shuttle Vectors Based on the Minimal pVS1 Replicon for Use in Gram-Negative, Plant-Associated Bacteria
• A Versatile Plasmid System for Translational Control and Secretion of Recombinant Proteins in Mycobacteria
• Construction of knockout mutants in Mycobacterium intracellulare ATCC13950 strain using a thermosensitive plasmid containing negative selection marker rpsL+
• Immunomagnetic recovery of Mycobacterium bovis from naturally infected environmental samples
• Mycobacterium intracellulare M.i.198—A Promising Strain for Studying Pathogenesis of Nontuberculous Mycobacterial Pulmonary Disease (NTM‐PD)