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Isolation and characterization of a potato cDNA corresponding to a 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene differentially activated by stress

2002/11/14 by María Eugenia Zanetti · 1 voice
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Plant Pathogenic Bacteria Studies #Plant Pathogens and Fungal Diseases #Plant-Microbe Interactions and Immunity

paper · doi:10.1093/jxb/

openalex publication_date 2002/11/14 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/15

Abstract

1-Aminocyclopropane-1-carboxylate (ACC) oxidase enzyme catalyses the final step in ethylene biosynthesis, converting 1-aminocyclopropane-1-carboxylic acid to ethylene. A cDNA clone encoding an ACC oxidase, ST-ACO3, was isolated from potato (Solanum tuberosum L.) by differential screening of a Fusarium eumartii infected-tuber cDNA library. The deduced amino acid sequence exhibited similarity to other ACC oxidase proteins from several plants species. Northern blot analysis revealed that the ST-ACO3 mRNA level increased in potato tubers upon inoculation with F. eumartii, as well as after treatment with salicylic acid and indole-3-acetic acid, suggesting a cross-talk between different signalling pathways involved in the defence response of potato tubers against F. eumartii attack.

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