2020/04/16 by Jennifer Moussa, Balig Panossian, Elie Nassour +3
Biochemistry, Genetics and Molecular Biology · #Antibiotic Resistance in Bacteria #Bacterial Genetics and Biotechnology #Bacterial Identification and Susceptibility Testing
paper · doi:10.1093/jac/dkaa181
openalex publication_date 2020/04/16 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
BACKGROUND: The spread of carbapenem-resistant Enterobacteriaceae is an important challenge and an increasing healthcare problem. OXA-48 is a class D carbapenemase that is usually localized on a conjugative plasmid belonging to the IncL incompatibility group. METHODS: In this study, we used a combination of short- and long-read WGS approaches and molecular typing techniques to characterize the genetic environment of the smallest reported 27 029 bp IncFII plasmid carrying blaOXA-48 (pLAU-OXA48). RESULTS: The plasmid recovered from a clinical Escherichia coli isolate was positive for blaOXA-48, which was located within the Tn6237 composite transposon. Primers targeting junctions between the IncF fragment and Tn6237 for the rapid identification of pLAU-OXA48-like plasmids were designed. CONCLUSIONS: To our knowledge, this is the first report showing the complete sequence of an IncFII plasmid carrying blaOXA-48 within Tn6237 using hybrid assembly of long- and short-read sequencing.