2003/09/01 by Tim Briggs, T. W. R. Briggs, S. Mahroof +6 · 2 citations
Medicine · #Knee injuries and reconstruction techniques #Osteoarthritis Treatment and Mechanisms
paper · pdf · doi:10.1302/0301-620x.85b7.13672
openalex publication_date 2003/09/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
We have performed a prospective, single-surgeon study analysing the histological results of autologous chondrocyte implantation. Fourteen patients underwent autologous chondrocyte implantation of the knee and were evaluated at one year by clinical assessment and arthroscopy. Standard staining was used to examine the sections. In addition, in situ hybridisation was used to establish type-IIa and type-IIb collagen mRNA expression and immunolocalisation techniques demonstrated the positions of type-II and type-X collagen. Eight patients regenerated hyaline cartilage and also contained type-X collagen in the deepest layers and type-II collagen in the deep layers. Three demonstrated fibrocartilage and had type-I collagen in the deep layers. In situ hybridisation revealed that all 14 samples had the potential to express both type-IIa and type-IIb collagen. We have shown that one year after the initial implantation chondrocytes are capable of producing type-II collagen and that they continue to proliferate and mature.