2015/07/30 by Éloi R. Verrier, Eloi R. Verrier, Che C. Colpitts +22 · 1 citation
Medicine · #Drug Transport and Resistance Mechanisms #Hepatitis B Virus Studies #Hepatitis C virus research
paper · pdf · doi:10.1002/hep.28013
openalex publication_date 2015/07/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
UNLABELLED: Chronic hepatitis B and D infections are major causes of liver disease and hepatocellular carcinoma worldwide. Efficient therapeutic approaches for cure are absent. Sharing the same envelope proteins, hepatitis B virus and hepatitis delta virus use the sodium/taurocholate cotransporting polypeptide (a bile acid transporter) as a receptor to enter hepatocytes. However, the detailed mechanisms of the viral entry process are still poorly understood. Here, we established a high-throughput infectious cell culture model enabling functional genomics of hepatitis delta virus entry and infection. Using a targeted RNA interference entry screen, we identified glypican 5 as a common host cell entry factor for hepatitis B and delta viruses. CONCLUSION: These findings advance our understanding of virus cell entry and open new avenues for curative therapies. As glypicans have been shown to play a role in the control of cell division and growth regulation, virus-glypican 5 interactions may also play a role in the pathogenesis of virus-induced liver disease and cancer.