2025/07/15 by Usha Kadiyala, David Sprinzak, Nick Monk +7 · 1 voice · 1 citation
Biochemistry, Genetics and Molecular Biology · #Gene Regulatory Network Analysis #Single-cell and spatial transcriptomics #Cell Image Analysis Techniques
paper · doi:10.1242/dev.204617
Developmental biology seeks to unravel the intricate regulatory mechanisms orchestrating the transformation of a single cell into a complex, multicellular organism. Dynamical systems theory provides a powerful quantitative, visual and intuitive framework for understanding this complexity. This Primer examines five core dynamical systems theory concepts and their applications to pattern formation during development: (1) analysis of phase portraits, (2) bistable switches, (3) stochasticity, (4) response to time-dependent signals, and (5) oscillations. We explore how these concepts shed light onto cell fate decision making and provide insights into the dynamic nature of developmental processes driven by signals and gradients, as well as the role of noise in shaping developmental outcomes. Selected examples highlight how integrating dynamical systems with experimental approaches has significantly advanced our understanding of the regulatory logic underlying development across scales, from molecular networks to tissue-level dynamics.