2002/06/01 by D. S. Buss, David S. Buss, A. R. Mccaffery +3
Medicine · #Biochemistry #Biology #Calcium #Culex #Drug Transport and Resistance Mechanisms #Ecology #Endosulfan #Enzyme #Immunology #Internal medicine #Larva #Malaria Research and Control #Malathion #Monocrotophos #Mosquito control #Pesticide #Pharmacological Effects and Toxicity Studies #Pharmacology #Toxicity #Toxicology #Verapamil #Xenobiotic
paper · doi:10.1046/j.1365-2915.2002.00365.x
openalex publication_date 2002/06/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/05/21
Pesticide resistance has parallels with multi-drug resistance syndrome of tumours in clinical medicine, which has been linked to an ATP-dependent pump, p-glycoprotein (P-gp). P-gps pump drugs out of the cell, thereby reducing cellular concentrations of the chemical. P-gps have been found in several invertebrate species and have been shown to provide a defence against environmental xenobiotics, including pesticides. This study used a model cell culture system to investigate the interaction of pesticides with P-gp. Ivermectin and endosulfan were shown to be strong inhibitors of dye transport out of cells, which is a standard measure of P-gp modulation. We then investigated the action of a P-gp inhibitor, verapamil (calcium channel blocker), on insecticide toxicity to fourth-instar mosquito larvae of the Culex pipiens L. complex (Diptera: Culicidae). Verapamil increased toxicity to examples of three insecticide classes (cypermethrin, endosulfan, ivermectin), but not to chlorpyrifos (organophosphate). The discovery of a novel protective mechanism in mosquitoes, with a wide substrate range, has implications for the control of important pest and vector species.