1975/07/01 by Murray Men-chung Tseng, Morris Wayman
Biochemistry, Genetics and Molecular Biology · #Microbial metabolism and enzyme function #Fungal and yeast genetics research #Microbial Metabolic Engineering and Bioproduction
paper · doi:10.1139/m75-147
Two Candida species and a Saccharomyces were grown on ethyl alcohol, acetic acid, ethyl acetate, and I-butanol, and their growth rates at a series of concentrations of each substrate are reported. Substrate concentrations higher than a characteristic threshold substrate concentration Stheta inhibited growth in accordance with a new relationship: mu = mumaxS/(k+S) - i(S - Stheta, where i is an inhibition constant and the other symbols have their usual meanings. Complete inhibition, that is, no growth, takes place when S = Si, the total inhibition concentration. Values of Stheta, Si, and i were calculated for the examples studied. Also discussed are the observed lag periods and also the lowered yields at high substrate concentrations.