1986/01/01 by Wade H. Bingle, Eldor A. Paul · 1 citation
Agricultural and Biological Sciences · Medicine · #Mycorrhizal Fungi and Plant Interactions #Fungal Biology and Applications #Plant and fungal interactions
paper · doi:10.1139/m86-012
When fungal hyphae were separated from two mineral soils by wet sieving and density gradient centrifugation, a preparation consisting of 50% of the total soil fungal hyphae and 3% of the soil dry weight was obtained. Residual soil particles were removed by an additional sieving step which resulted in a final recovery of 20% of the fungal hyphae from soil. Scanning electron microscopy revealed that some soil particles still contaminated the hyphae but the exact amount of soil could not be determined by dry weight measurements. These data indicated that reasonable separation of fungal hyphae from soil for use in biomass (i.e., nutrient analysis) studies is feasible.