2025/04/01 by Estela Giménez, Eliseo Albert, Ester Colomer +9
Medicine · #Cytomegalovirus and herpesvirus research #Herpesvirus Infections and Treatments #Mosquito-borne diseases and control
paper · pdf · doi:10.1002/jmv.70355
openalex publication_date 2025/04/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
ABSTRACT We compared the performance of the VIDAS Cytomegalovirus (CMV)‐Interferon‐gamma release assay (IGRA) with that of laboratory‐developed flow cytometry for intracellular cytokine staining (FC‐ICS) for the assessment of CMV‐specific interferon‐gamma (IFN‐γ)‐producing T‐cell responses (CMV‐CMI). A total of 147 blood specimens from 78 adult participants were collected: 11 healthy controls, 34 hematological patients (HP), of which 32 had undergone allogeneic hematopoietic cell transplantation (allo‐HCT), and 33 Kidney transplant recipients. Of the 147 specimens, 96 tested positive, 24 negative, 25 indeterminate, and 2 were invalid by the VIDAS CMV IGRA. A total of 137 specimens were tested by FC‐ICS, of which 107 returned positive results. There were 27 discrepancies across the assays among specimens yielding interpretable results, of which 14 tested VIDAS CMV IGRA‐positive/FC‐ICS‐negative and 12 VIDAS CMV IGRA‐negative/FC‐ICS‐positive. The overall agreement between immunoassays was 78%, and the Kappa coefficient was 0.34 (0.52 for HP). Differences in identifying CMV‐infected (CMV IgG‐positive) and uninfected participants (CMV IgG‐negative) were noticed across both assays. The overall correlation (rho values) between IFN‐γ concentrations (IU/ml) measured by the VIDAS CMV IGRA and CMV‐specific IFN‐γ‐producing T‐cell frequencies were 0.27 for CD4 + and 0.33 for CD8 + T cells ( p = 0.001). In HP, the correlation was stronger (0.48 for CD4 + and 0.49 for CD8 + T cells). In summary, our data lend support to the potential utility of the VIDAS CMV IGRA to assess CMV‐CMI in transplant recipients.