Pulsatile microvascular blood flow imaging by short-time Fourier transform analysis of ultrafast laser holographic interferometry

2015/10/07 by Leo Puyó, Isabelle Férézou, Puyo, L. +13
Engineering · Medicine · #FOS: Physical sciences #Medical Physics (physics.med-ph) #Optical Imaging and Spectroscopy Techniques #Optics (physics.optics) #Photoacoustic and Ultrasonic Imaging #Thermoregulation and physiological responses

paper · pdf · doi:10.48550/arxiv.1510.01892

openalex publication_date 2015/10/07 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

We report on wide-field imaging of pulsatile microvascular blood flow in the exposed cerebral cortex of a mouse by holographic interferometry. We recorded interferograms of laser light backscattered by the tissue, beating against an off-axis reference beam with a 50 kHz framerate camera. Videos of local Doppler contrasts were rendered numerically by Fresnel transformation and short-time Fourier transform analysis. This approach enabled instantaneous imaging of pulsatile blood flow contrasts in superficial blood vessels over 256 x 256 pixels with a spatial resolution of 10 microns and a temporal resolution of 20 ms.

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