2006/01/01 by Andreas Tholey, Tholey, Andreas
Biochemistry, Genetics and Molecular Biology · Chemistry · #Advanced Proteomics Techniques and Applications #MALDI #Mass Spectrometry Techniques and Applications #Metabolomics and Mass Spectrometry Studies #ionic liquid matrices #mass spectrometry #quantification
paper · doi:10.4230/dagsemproc.05471.10
openalex publication_date 2006/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
The determination of absolute protein amounts and the quantification of differentially expressed proteins belong to the most important goals in proteomics. Despite being one of the key technologies for the identification of proteins, the application of matrix assisted laser desorption/ionization (MALDI) mass spectrometry (MS) for quantitative analyses is hampered by several inherent factors. The goal of the present paper is to outline these difficulties but also to present some selected approaches which enable MALDI MS to be used for the quantification of biomolecules. In particular, methods for the improvement of the homogeneity of MALDI samples and the use of internal standards for the relative quantification are discussed. Strategies for in-vivo and in-vitro labelling of peptides and proteins with stable isotopes are presented. The need for guidelines for the presentation and evaluation of data as well as for bioinformatical approaches for the interpretation of quantitative data will be addressed.