2014/01/01 by Rutger Matthes, Matthes, Rutger, Ojan Assadian +3
Biochemistry, Genetics and Molecular Biology · Medicine · #610 Medical sciences #Bacillus and Francisella bacterial research #Medicine #Microbial Inactivation Methods #Plasma Applications and Diagnostics #cold atmospheric pressure plasma #resistance development
paper · doi:10.3205/dgkh000237
openalex publication_date 2014/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/19
INTRODUCTION: The increasing microbial resistance against antibiotics complicates the therapy of bacterial infections. Therefore new therapeutic options, particularly those causing no resistance, are of high interest. Cold atmospheric plasma is one possible option to eradicate multidrug resistant microorganisms, and so far no resistance development against physical plasma is known. METHOD: We tested 6-fold repeated plasma applications on a Staphylococcus aureus strain embedded in biofilm and compared the reduction of the colony forming units between the different treatment periods to asses a possible development of resistance. RESULT: For all treatment periods, the control biofilms were reduced by plasma in average by 1.7 log10 CFU, and decreased from 7.6 to 5.8 log10 (CFU/cm(2)) within 5 hours. The results demonstrated that repeated plasma doses not induce resistance or habituation against plasma applied within short time periods. CONCLUSION: The repeated application of cold plasma is a promising option for the treatment of infected wounds without the risk of development of resistance against plasma.