2017/01/01 by Drevon, Jean-Jacques, Jean‐Jacques Drevon
Agricultural and Biological Sciences · Materials Science · #0 #Bio-geochimical cycles #Carence en P #Clay minerals and soil interactions #Cycles bio-géochimiques #In situ RT-PCR #Lignées recombinantes #Marqueur moléculaire #Molecular marker #Nodule #P-deficiency #Phaseolus vulgaris #Phytase and its Applications #Plant Pathogens and Resistance #RT-PCR in situ #Recombinant inbred lines
paper · doi:10.15454/1.5138504822863843e12
openalex publication_date 2017/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/15
Decreasing chemical inputs in agriculture, like fertilizers or pesticides, becomes of paramount importance for the nutritional safety and environmental impact of food productions. This implies new requirements with regard to the selection of legume cultivars for cropping- systems and their beneficial interactions with soil microbes. Low phophorus availability in about 40% of the world’s arable land limits crop yield, most particularly for legume crops when their growth depends upon symbiotic N2 fixation (SNF). Therefore, our work aims to increase the phosphorus use efficiency (PUE) for SNF, and its contribution to a more effective coupling between the P and N bio-geochemical cycles. Myo-inositol hexakisphosphate, i.e. phytate, constitutes a major source of organic P (Po) in soils, but is unavailable to plants. Phytases are the only phosphatases able to hydrolyse phytate efficiently into inorganic P (Pi), thus increasing soil-P bio-availability for plants. In the FABATROPIMED project we have demonstrated the existence of phytases, both histidine acid and beta-propeller among rhizobia nodulating Vicia faba and Vigna unguiculata, and shown by in situ RT-PCR, their expression within nodule infected-cells. Phytase mRNA of the host-legume was also localized in nodules and shown to increase under P deficiency with significant variation among recombinant inbred lines of Phaseolus vulgaris which are contrasting in their PUE for SNF. A subsequent virtuous cycle of P and N fertility of soils is being tested through a participatory approach with farmers in reference agroecosystems offering a wide range of agroclimatical and socioecological situations. It is concluded that the interdisciplinary research strategy FTM may increase the benefit of grain-legumes for cereal systems and the environment, by better understanding the interactions between soil microbes and plants to acquire and use N and P most efficiently.