Uptake of fluorescent nano beads into BY2‐cells involves clathrin‐dependent and clathrin‐independent endocytosis

2012/08/27 by Vera Bandmann, Jasmin Daniela Müller, Jasmin Müller +2 · 30 citations
Biochemistry, Genetics and Molecular Biology · Engineering · #Cellular transport and secretion #Plasmonic and Surface Plasmon Research #Plant Reproductive Biology

paper · doi:10.1016/j.febslet.2012.08.008

Abstract

To follow endocytosis in BY-2 cells we made use of fluorescent nano beads. Beads with 20nm in diameter were internalised rapidly and accumulated partially in compartments also labelled by the endocytic marker FM4-64. Studies in BY-2 cells and protoplasts revealed that larger beads (100nm) were excluded from uptake into turgescent and plasmolysed cells while protoplasts were able to internalise beads with a diameter of up to 1000nm. Endocytosis of beads was only partially inhibited by the clathrin-specific inhibitor Ikarugamycin and strongly blocked by wortmannin. These results imply that uptake of beads involves clathrin-dependent and clathrin-independent endocytic mechanisms and supports the hypothesis that clathrin-independent endocytosis plays a general role in plants.

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