BMP signaling regulates Nkx2‐5 activity during cardiomyogenesis

2001/11/27 by Mina Jamali, Christina Karamboulas, Parker J Rogerson +2 · 13 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Congenital heart defects research #Muscle Physiology and Disorders #Cardiomyopathy and Myosin Studies

paper · pdf · doi:10.1016/s0014-5793(01)03151-9

Abstract

Nkx2-5 regulates the transcription of muscle-specific genes during cardiomyogenesis. Nkx2-5 expression can induce cardiomyogenesis in aggregated P19 cells but not in monolayer cultures. In order to investigate the mechanism by which cellular aggregation regulates Nkx2-5 function, we examined the role of bone morphogenetic protein 4 (BMP4). We showed that the expression of the BMP inhibitor, noggin, was sufficient to inhibit the induction of cardiomyogenesis by Nkx2-5 during cellular aggregation. Furthermore, soluble BMP4 could activate Nkx2-5 function in monolayer cultures, resulting in the formation of cardiomyocytes. Therefore, BMP signaling is necessary and sufficient for the regulation of Nkx2-5 activity during cardiomyogenesis in P19 cells.

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• BMP signaling in congenital heart disease: new developments and future directions. [europepmc]
• Mechanisms of cardiogenesis in cardiovascular progenitor cells. [europepmc]
• Tbx6 is a determinant of cardiac and neural cell fate decisions in multipotent P19CL6 cells. [europepmc]
• Tissue engineering approaches to heart repair. [europepmc]
• Combined effects of bone morphogenetic protein 10 and crossveinless-2 on cardiomyocyte differentiation in mouse adipocyte-derived stem cells. [europepmc]
• Hsa-miR-335 regulates cardiac mesoderm and progenitor cell differentiation. [europepmc]
• Xenopus : Experimental Access to Cardiovascular Development, Regeneration Discovery, and Cardiovascular Heart-Defect Modeling. [europepmc]
• TGFβ Family Signaling Pathways in Pluripotent and Teratocarcinoma Stem Cells' Fate Decisions: Balancing Between Self-Renewal, Differentiation, and Cancer. [europepmc]
• Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development. [europepmc]
• The benign nature and rare occurrence of cardiac myxoma as a possible consequence of the limited cardiac proliferative/ regenerative potential: a systematic review. [europepmc]

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