Nitric oxide and proteoglycan biosynthesis by human articular chondrocytes in alginate culture

1994/10/03 by Hans Jörg Häuselmann, H.J. Häuselmann, L. Oppliger +6 · 7 citations
Medicine · Biochemistry, Genetics and Molecular Biology · #Osteoarthritis Treatment and Mechanisms #Nitric Oxide and Endothelin Effects #Proteoglycans and glycosaminoglycans research

paper · doi:10.1016/0014-5793(94)00994-5

Abstract

Interleukin-1 alpha and beta induced the production of large amounts of nitric oxide by normal, human articular chondrocytes in alginate culture; at the same time the biosynthesis of proteoglycan was strongly suppressed. In a dose-dependent manner, NG-monomethyl-L-arginine both inhibited nitric oxide formation and relieved the suppression of proteoglycan synthesis. However concentrations of NG-monomethyl-L-arginine which completely prevented nitric oxide production only partially restored proteoglycan biosynthesis, even at low doses of interleukin-1 where suppression of proteoglycan synthesis was modest. The organic donor of nitric oxide, S-nitrosyl-acetyl-D,L- penicillamine also inhibited proteoglycan biosynthesis, but not as extensively as interleukin-1. These data suggest that interleukin-1 suppresses synthesis of the cartilaginous matrix through more than one mechanism, at least one of which is dependent upon the production of nitric oxide.

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