Versteckte Vielfalt : Diversität von Zellen im industriellen Bioprozess

2023/08/04 by Arulrajah, Prasika, Heins, Anna-Lena
#Biology #Life Sciences

paper · doi:10.15480/882.8093

Abstract

Population heterogeneity poses a major challenge in industrial scale bioprocesses, affecting process performance but also leading to more robust phenotypes. To investigate this phenomenon, multiple fluorescent reporter strains are cultivated under simulated industrial scale conditions in multi-compartment bioreactors. This approach significantly raises the quantitative understanding of population heterogenity which is needed before its benefits can help to develop high-yielding, more robust bioprocesses.

Related

• Development and characterization of Escherichia coli triple reporter strains for investigation of population heterogeneity in bioprocesses
• Physiological heterogeneities in microbial populations and implications for physical stress tolerance.
• Influence of varying pre-culture conditions on the level of population heterogeneity in batch cultures with an escherichia coli triple reporter strain
• Application of an Escherichia coli triple reporter strain for at-line monitoring of single-cell physiology during L-phenylalanine production
• Impact of mixing insufficiencies on L-phenylalanine production with an Escherichia coli reporter strain in a novel two-compartment bioreactor