2016/01/06 by Clément Apelian, Fabrice Harms, Apelian, Clement +5 · 3 citations
Biochemistry, Genetics and Molecular Biology · Engineering · #Advanced Fluorescence Microscopy Techniques #Biological Physics (physics.bio-ph) #FOS: Physical sciences #Medical Physics (physics.med-ph) #Optical Coherence Tomography Applications #Optics (physics.optics) #Photoacoustic and Ultrasonic Imaging
paper · pdf · doi:10.48550/arxiv.1601.01208
openalex publication_date 2016/01/06 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/28
We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other imaging techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions.